摘要
目的研究一种病理进程平稳的急性胰腺炎胰腺外多器官损伤动物模型。方法应用小剂量中等浓度胆盐、胰管内低压注射的方法,动态观察实验大鼠胰腺、肺、肝脏及肾脏的功能和病理改变。结果这种新的急性坏死性胰腺炎大鼠模型具有胰腺外持续、稳定的肝、肾损伤。结论这种急性坏死性胰腺炎大鼠模型适合于多器官功能损伤的病理机制及治疗学研究。
Objective: To explore a new severe but sublethal acute pancreatitis model for the study of clinically relevant extrapancreatic multiorgan injury. Methods: The animals were divided into two groups: the bile acid injection group and the control group. In the bile acid injection group, acute necrotizing pancreatitis was induced by intraductal administration of 0.2 ml of 2.0 % bile acid under 30 cm H2O pressure, while the controls underwent the sham operation. The two groups were divided into six subgroups (8 rats for each) and sacrificed at 12, 24, 36, 48, 72 and 144 hours, respectively. The structural and functional features in the pancreas, lung, liver and kidney were examined. Results: Accompanied with acute necrosis pancreatitis, the functional and histologic changes had developed continuously in the kidney and liver for 72 and 144 hours in the bile acid injection animals respectively. No pancreatitis associated pulmonary changes were found. Conclusions: The sublethal acute pancreatitis model is suited for pathogenetic and therapeutic study of liver and renal changes in acute necrotizing pancreatitis.
出处
《中国现代医学杂志》
CAS
CSCD
2004年第15期53-55,共3页
China Journal of Modern Medicine
关键词
急性胰腺炎
动物模型
器官功能衰竭
acute experimental pancreatitis
model
multiorgan injury
