摘要
目的 对先天性肛门直肠畸形 (congenitalanorectalmalformations,CAM )末端直肠组织Hoxc 8基因表达水平的测定以探讨其分子水平的发病机制。方法 应用RT PCR(逆转录 聚合酶链反应 )技术检测 32例CAM的末端直肠组织及 8例对照组直肠标本的Hoxc 8基因表达 ,以 β actin基因 (是一种在组织中表达稳定的基因 )作为阳性内对照 ,观察Hoxc 8基因的表达水平。结果 CAM组Hoxc 8表达的相对比值为 0 .892± 0 .0 6 8,对照组则为 1.0 0 6± 0 .0 82。Hoxc 8在CAM组的表达与对照组比较差异有非常显著性意义 ,P <0 .0 1。结论 CAM的发生与Hoxc 8基因密切相关 ,该基因在肛门直肠组织中表达减弱可能参与导致其相应的靶器官发育异常而产生畸形。
Objective To investigate the relationship between the expression of Hoxc-8 and the pathogenesis of congenital anorectal malformations (CAM).Methods The Hoxc-8 expression in rectum of 32 CAM and 8 patients with other diseases were studied by reverse transcription-polymerase chain reaction( RT-PCR).β-actin was included as control.Results The relative ratio of the expression of gene Hoxc-8 was 0.892 ± 0.068 ,whereas that of the control was 1.006 ± 0.082 . The expression of gene Hoxc-8 in CAM was significantly lower than that of the control ( P < 0.01 ).Conclusions The pathogenesis of CAM is closely related with Hoxc-8. Its weaker expression in anorectum may play a role in dysplasia of its corresponding target organs and result in malformations.
出处
《中华小儿外科杂志》
CSCD
北大核心
2004年第3期258-260,共3页
Chinese Journal of Pediatric Surgery
