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戊二醛固定剂对海洋微微型浮游植物特性的影响 被引量:2

The Impact of Glutaraldehyde as Fixative on Marine Picophytoplankton
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摘要 试验比较了新鲜样品和经1%戊二醛固定液氮保存的样品在细胞密度、细胞大小、自发荧光强度等参数变化,结果表明,经戊二醛固定处理后,3种微微型浮游植物(原绿球藻、聚球藻和微型真核浮游植物)的细胞密度都有不同程度的增加;原绿球藻和聚球藻的细胞大小有不同程度的增大,而微型真核浮游植物则减小;三者的自发荧光强度都减弱. Samples are fixed with 1% glutaraldehyde and stored in liquid Nitrogen . Cell density 、 size and intensity of autofluorescence are compared with fresh samples in this experiment . The results showed that cell density and cell size of all three picophytoplanktonic populations (prochlorococcus, synechococcus and picoeukaryotes) increased, but intensity of autofluorescence decreased.
作者 张利华 晁敏
出处 《华东师范大学学报(自然科学版)》 CAS CSCD 北大核心 2004年第3期93-98,共6页 Journal of East China Normal University(Natural Science)
基金 国家重点基础研究专项经费资助项目(G19990437)
关键词 流式细胞术 戊二醛 微微型浮游植物 flow cytometry glutaraldehyde picophytoplankton
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