摘要
试验用随机扩增多态DNA(RAPD)技术对黄鳝属中产自缅甸的山黄鳝、印度尼西亚的穴黄鳝及中国的黄鳝的混合DNA进行引物筛选。40条引物有35条引物扩增出多态性片段。35条引物共获得334个扩增片段,长度为250～5417bp。
The samples of three species of synbranchi form eels,Monopterus cuchia,M.fossorius and M.albus were collected from Yangon of Myanmar,Indonesia and Yuanjiang County, Hunan Province,China respectively.Randomly amplified polymorphic DNA (RAPD) technique was used to analyse the selection of polymorphic primers of the wild grouper population of four individuals from each species.35 out of 40 polymorphic priners amplified diversity.35 primers produced 334 fingerprints with the molecular length varying from 250 bp to 5417 bp.
出处
《水产科学》
CAS
北大核心
2004年第9期23-25,共3页
Fisheries Science
关键词
山黄鳝
穴黄鳝
黄鳝
RAPD
引物筛选
Monopterus cuchia
M.fossorius
M.albus
RAPD
selection of polymorphic primers
