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隆肛蛙卵巢性类固醇激素的免疫细胞化学研究 被引量:5

A study on immunocytochemistry of sex steroid hormones of ovary of the Rana quadranus
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摘要 目的 深入探讨两栖动物卵巢内不同性类固醇激素在卵子发育中的调控作用。方法 用免疫细胞化学方法对隆肛蛙不同发育时期卵泡的性类固醇激素雌二醇、孕酮和睾酮进行定位检测。结果 雌二醇在卵黄合成期的卵母细胞和滤泡细胞中表达为强阳性,而在趋于成熟的卵泡中表达减弱;孕酮在卵黄合成期和卵黄合成后期的卵母细胞和滤泡细胞中表达较强;睾酮在各期卵母细胞中的表达均较弱,仅在卵黄合成后期表达略有增强。结论 雌二醇与卵黄合成关系密切,而睾酮和孕酮在卵母细胞成熟中起着重要的调控作用。 Aim It is researched deeply that sex steroids regulate the oocyte development of amphibian.Methods Immunocytochemicallocalizationwereinvestigatedinthedifferentdevelopmentalstagesofthefolli clesoftheRanaguadranususingantibodiesagainst 17β estradiol,progesteroneandtestosterone .Results 17β estradiolisexpressedstronglyintheoocytesandthefolliclecellsatthevitellogenicstages,butweakenedatthe postvitellogenicstage .Progesteroneisexpressedstronglyintheoocytesatthevitellogenicandpostvitellogenicstage .Immunopositivereactiontotestosteroneantibodiesintheoocytesstrengthenednotablyatpostvitellogenic stage .Conclusions 17β estradiolisconcernedwiththesynthesisofyolkprotein .Progesteroneandtestosterone regulateoocytematurationoftheRanaquadranus.
出处 《西北大学学报(自然科学版)》 CAS CSCD 北大核心 2004年第4期446-448,464,i001,共5页 Journal of Northwest University(Natural Science Edition)
基金 陕西省自然科学基金资助项目(2001SM26)
关键词 隆肛蛙 性类固醇激素 免疫细胞化学 Rana guadranus sex steroid hormone immunoc ytoche mistry
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