摘要
目的 检测硫芥对小鼠淋巴瘤L5 178Y3 .7.2c tk+ - 细胞的诱变性。方法 采用 96孔微孔板接种法检测平板接种效率和突变频率。结果 硫芥可诱导L5 178Y3 .7.2c tk+ - 细胞tk位点的突变 ,诱发突变是自发突变的 2～ 15倍。在tk位点诱发了大克隆和小克隆两种不同表型的突变集落 ,以小克隆为主。结论 硫芥有明确的致突变性和较强的细胞毒性 。
Objective To detect the mutagenicity of sulfur mustard in the tk gene of L5178Y3.7.2c tk +/- mouse lymphoma cells. Methods The plating efficiency and the mutant frequency were detected by the microtiter procedure. Results Sulfur mustard induced tk locus mutation with mutation frequency 2-15 times higher than that of spontaneous mutation frequency of L5178Y3.7.2c tk +/- cells. There were two different phenotypes of mutation colonies induced, which were large and small colonies, but most of them were small colonies. Conclusion Sulfur mustard is characterized by marked mutagenic activity and cytotoxicity. The compound may result in large range of DNA damage.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2004年第16期1426-1428,共3页
Journal of Third Military Medical University
基金
全军医学科学技术研究"十五"计划指令性课题 ( 0 1L0 54 )~~
关键词
TK
基因
突变
硫芥
tk
gene
mutation
sulfur mustard
