摘要
将ACLSVCP基因克隆到表达载体pET 28a(+)中,转化大肠杆菌BL21,筛选得到阳性克隆pET ACP,用IPTG诱导使其表达,SDS PAGE电泳分析表明,预期的22kD外壳蛋白在大肠杆菌中得到大量表达,并加以纯化,用于制备抗血清。
The coat protein gene of ACLSV was cloned into expression vector pET-28a(+),then translated into E.coli BL21,and the recombinant plasimid was obtained.ACLSV CP gene was expressed in E.coli through IPTG inducing.A 22kDa peculiar protein as expected was shown in SDS-PAGE.The expressed protein was pufied and it will be used for preparing antiserum.
出处
《石河子大学学报（自然科学版）》
CAS
2004年第4期295-297,共3页
Journal of Shihezi University(Natural Science)
基金
国家科技攻关计划课题(2003BA546C)
