摘要
目的 :探讨以同种异体的软骨细胞作为种子细胞、以聚乳酸 (PLA)作为支架 ,应用组织工程学方法在体内形成新生软骨组织的可能性 ,为组织工程软骨修复喉、气管软骨缺损提供实验依据。方法 :将出生 3～ 5d的新西兰大白兔乳兔四肢关节软骨组织酶解 ,分离出软骨细胞 ,体外扩增后 ,将其接种到PLA三维可吸收支架上 ;将软骨细胞 PLA复合物移植到成年新西兰大白兔的背部皮下 ,经过 7周的体内培养 ,将组织标本行大体观察和组织学检查。结果 :软骨细胞 PLA复合物在同种异体的动物体内有新生组织形成 ,经组织学染色 ,证实为软骨组织 ;随着植入时间的延长 ,软骨基质的分泌增加 ,炎性细胞的浸润逐渐减轻。结论 :同种异体的软骨细胞 PLA复合物在有免疫力的动物体内能够形成新生软骨组织 ,这一方法为喉、气管软骨缺损的修复提供了一条新的途径。
Objective:To explore the feasibility of producting neocartilage tissue by using allogenic chondrocytes as seeding cells, artificial absorbable scaffold-polylactic acid(PLA) as matrices.Method:The cartilage tissue was obtained from the four limbs of neoborn New Zealand rabbits(born 3-5 days), digested by Ⅱ collagenase, proliferated in vitro,then seeded the chondrocytes on PLA scaffolds. The chondrocyte-PLA complex were cultured 7-10 days in vitro, then implanted in dorsal subcutaneous of adult New Zealand rabbits. Specimens harvested 3 weeks、5 weeks and 7 weeks after implantation respectively and subjected to histologic analysis.Result:Chondrocyte-PLA complex implanted in adult new Zealand rabbits for 7 weeks, producted neo-cartilage as proved by histologic analysis, along with time extending, chondrocyte matrice secretion increased, invading inflammatory cell decreased.Conclusion:Using allogenic chondrocytes as seeding cells and PLA scaffold,it is successful to generate allogenic tissue-engineering cartilage in animals that have inherent immunity.
出处
《临床耳鼻咽喉科杂志》
CSCD
北大核心
2004年第8期485-487,共3页
Journal of Clinical Otorhinolaryngology
关键词
组织工程
同种异体
软骨细胞
聚乳酸
Tissue-engineering
Allogenic
Chondrocyte
Polylactic acid
