摘要
目的 :观察依立雄胺在体外对激素非依赖型人前列腺癌细胞 (PC3)正常生长及Bcl 2蛋白表达的作用。方法 :用 5 ,15和 4 5 μmol·L- 1的依立雄胺作用PC3细胞 3d或 7d后 ,相差显微镜进行细胞形态学观察 ;苔盼蓝染色活细胞计数 ,绘制 7d的生长曲线 ;应用流式细胞仪分析依立雄胺对PC3细胞凋亡的影响 ;应用免疫组化比较经依立雄胺作用后PC3细胞Bcl 2的表达强度。结果 :依立雄胺在5 ,15和 4 5 μmol·L- 1作用PC3细胞 3d后 ,可使其有明显的皱缩脱壁 ,部分细胞膜破裂 ,并抑制PC3细胞的生长 ,抑制率依次为 19% ,2 6 %和 6 0 % ;流式细胞仪分析显示上述浓度的依立雄胺不诱导细胞凋亡 ,但可抑制细胞于DNA合成 (S)期 ,其百分率分别为 18% ,16 %和 11% ,而对照组为 33% ;免疫组化分析表明依立雄胺也不能降低PC3细胞中Bcl 2的表达。结论 :5 ,15和 4 5 μmol·L- 1的依立雄胺在体外可抑制PC3细胞的生长 ,机制可能为抑制细胞DNA的合成。
AIM:To observe the effect of epristeride on the growth of human androgen-independent prostatic cancinoma cells (PC3) and the expression of Bcl-2 protein in vitro . METHODS:The inhibitory effect on the prostate carcinoma cells treated with epristeride 5,15,and 45 μmol·L -1 for 3 d or 7 d was morphologically observed and the growth curves were drawn on a plot of cells versus time. DNA flow cytometry to detect apoptosis and immunohistochemical staining for Bcl-2 was used to explore the possible mechanisms in the epristeride concentrations of 5,15,and 45 μmol·L -1 . RESULTS:Epristeride inhibited the growth human prostatic cancer cell line of PC3 with the inhibition rate of 19 %,26 % and 60 % when treated with epristeride for 3 d in the concentrations of 5,15 and 45 μmol·L -1 ,respectively. The epristeride in the three concentrations didn't induce apoptosis in the PC3 cells but inhibited the cells in DNA systhesis (S) phase,the percentages of S phase were 18 %,16 % and 11 %,respectively. The percentages of S phase in the control group was 33 %. The epristeride couldn't dose-dependently decrease the expression of Bcl-2 in the PC3 cells. CONCLUSION:Eprsiteride is able to inhibit the growth of human prostate carcinoma cell line PC3 in the concentrations of 5,15 and 45 μmol·L -1 in vitro . The mechanisms may be related to it's inhibiting the cells in S phase.
出处
《中国新药与临床杂志》
CAS
CSCD
北大核心
2004年第8期485-488,共4页
Chinese Journal of New Drugs and Clinical Remedies
基金
上海市重点科学技术发展基金资助(0 0JC14 0 4 5 )
