摘要
目的 探讨辐射对转染pEgr MIP3α质粒的Lewis肺癌细胞MIP3α表达的影响。方法 用PCR方法扩增出MIP 3α基因 ,构建 pEgr MIP3α重组表达质粒 ,脂质体介导转染Lewis肺癌细胞 ,用RT PCR和Western方法检测不同剂量X射线照射后的MIP 3α表达水平。结果 所构建的重组质粒 pEgr MIP3α中MIP 3αcDNA正确插入表达载体 ;各照射组在不同剂量电子线照射后 ,MIP 3α表达均高于未转染组和假照射组。结论 Egr 1启动子具有辐射启动和诱导下游MIP 3α基因在Lewis肺癌中增强表达的功能 ,为pEgr MIP3α用于放射
Objective Cloning MIP 3α cDNA and constructing pEgr MIP3α expression vector to study the expression of MIP 3α in Lewis lung cancer cells transfected with the expression vector.Methods MIP 3α cDNA was cloned by PCR and inserted into plasmid with Egr 1 promoter.The vectors were transfected into Lewis lung cancer cells with liposomes.The expression of MIP 3α in the transfected Lewis lung cancer cells with electron ray irradiation in different doses were confirmed by RT PCR and Western blot analysis.Results The inserted sequence in the recombined vector was correct when it was sequenced.After electron ray irradiation in different doses,MIP 3α expression in the transfected Lewis lung cancer cell increased significantly.Conclusion Egr 1 promoter can induce MIP 3α expression in Lewis lung cancer cells transfected with the recombine plasmid pEgr MIP3α when the cells were radiated with electron ray in different doses.
出处
《重庆医学》
CAS
CSCD
2004年第7期968-970,共3页
Chongqing medicine
基金
国家自然科学基金资助项目 (30 30 0 1 50 )
