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基因治疗的新武器——RNA干扰技术的应用现状 被引量:2

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摘要 由双链RNA介导的RNA干扰 (RNAinterference ,RNAi)又称为转录后基因沉默 (posttranscriptionalsi lencing ,PTGS) ,既能对抗如病毒基因或人工转入基因所表达的mRNA等外源性基因的侵害 ,又能降解自身异常基因所产生的mRNA ,具有特异、高效、操作简单的特点。国际上已开始利用RNA干扰技术分析结构基因的功能 ,调控细胞周期 ,调节细胞代谢 ,并且应用这种特异性沉默靶基因的技术抑制病毒如爱滋病病毒、肝炎病毒和疱疹病毒以及各种癌基因、癌相关基因或突变基因的过度表达 ,从而有望为疾病的基因治疗提供新策略。
出处 《国外医学(生理病理科学与临床分册)》 2004年第4期379-381,共3页 Foreign Medical Sciences(Pathophysiology and Clinical Medicine)
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  • 1lbashir SM, Harborth J, Lendeckel W,et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells[ J ]. Nature ,2001,411 ( 6836 ) :494-498. 被引量:1
  • 2Hammond SM, Bernstein E, Beach D,et al. An RNA-direcled nuclease mediates post-transcriptional gene silencing in Drosophila cells[ J ], Nature,2000,404 ( 6775 ) :293-296. 被引量:1
  • 3Guo S,Kemphues KJ. par-I ,a gene required for estnblishing polarity in C. Elegans embryos,encodes a putative Ser/Thr kinase that is asymmetrically distributed [ J ]. Cell, 1995,81 ( 4 ) :611 .-620. 被引量:1
  • 4Novina CD,Murray MF,Dykxhporn DM,et al. siRNA-directed inhibition of HIV-Ⅰ infection [ J ]. Nat Med,2002,8 ( 7 ) :681-686. 被引量:1
  • 5Surabhi RM, Gaynor RB. RNA interference directed against viral and cellular targets inhibits human immunodeficiency virus typel replication [ J ]. J Viro1,2002,76 ( 24 ) : 12963-12973. 被引量:1
  • 6Lee NS, Dohjima T, Bauer G,et al. Expression of small interfering RNAs targeted against HIV-I rev transcripts in human cells[ J ]. Nat Biotechno1,2002,20( 5 ) :500-505. 被引量:1
  • 7Shlnmai A, Shaul Y. Inhibition of Hepatitis B virus expression and replication by RNA interference [ J ]. Hepatology ,2003,37 ( 4 ) : 764-770. 被引量:1
  • 8Randall G,Grakoui A ,Rice CM. Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs [ J ].Proc, Natl Acad Sci USA,2003,100( 1 ) :235-240. 被引量:1
  • 9Kapadia SB, Brideau-Andc~en A, Chisari FV. Interference of hepatills C virus RNA replication by short interfering RNAs I J ]. Proc Natl Acad Sci USA ,2003, lO0( 4 ) :2014-2018. 被引量:1
  • 10Jia Q,Sun R. Inhibition of gammaherpesvirus replication by RNA interference[ J ]. J Viro1.2003,77 ( 5 ) :3301-3306. 被引量:1

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