摘要
本文利用人红细胞膜蛋白内源荧光(主要指色氨酸),经加入不同的竹红菌乙素之后,引起了荧光猝灭现象,根据荧光猝灭原理分别从吸收光谱,荧光光谱,稳态变温实验和瞬态荧光等技术分析了上述的猝灭过程,实验证明:该猝灭是以动态碰撞过程为主要作用机理,据此作者提出了乙素可以用于生物膜体系的蛋白质荧光猝灭剂的理由。
In this paper, we stuied the quenching mechanism of the fluorescence of erythrocte membrane by Hypocrellin B (HB). The intrinsic fluorescence can be quenched both by Dynamic and Static quenching. The kinetic analysis of the data indicated that the dynamic quenching is a process of a charge transfer. The static quenching may be from exciplexer between HB and tryptophan. Above experiments illustaed that HB can be a new quencher of protein fluorescence in biomembranes.
出处
《感光科学与光化学》
CSCD
1993年第3期228-235,共8页
Photographic Science and Photochemistry
基金
国家自然科学基金
生物物理所所长基金
关键词
红细胞膜
蛋白
竹红菌乙素
fluorescence quenching, erythrocyte membrane, Hypocrellin B
