摘要
目的:探讨大鼠胸主动脉内膜剥脱后内膜增生时缬沙坦对血管平滑肌细胞增殖、C-myc及p53基因表达的影响。方法;42只大鼠随机分为假手术组6只,单纯损伤组与损伤+药物治疗组(简称药物治疗组)各18只。药物治疗组术前6天至术后14天给予缬沙坦20mg·kg-1·d-1,余2组不给药。术后从7、14天取材。应用免疫组化方法测定PCNA、C-myc、p53蛋白在血管中的表达。结果:内膜损伤后,术后14天药物治疗组的内膜面积与单纯损伤组相比,差异有显著性。药物治疗组中PCNA和C-myc癌基因的表达均低于单纯损伤组,而抑癌基因p53的表达高于单纯损伤组。结论:缬沙坦可能通过抑制血管平滑肌细胞的增殖、C-myc癌基因的表达,升高抑癌基因p53的表达,而防治血管再狭窄。
Objective: To investigate the influence of valsartan on the proliferation of vascular smooth muscle cells and expression of C-myc oncogene and P53 tumor suppressor gene after balloon injury in thoracic aorta of rats. Methods: Forty-two rats were divided randomly into three groups: sham operation group (n=6), injury group (n=18) and medical treatment group (valsartan+injury) (n=18). Valsartan (20 mg·kg-1·d-1 P. O. ) was given to 18 rats in medical treatment group from 6 days before to 14 days after balloon injury. At the second, 7th, 14th day following balloon injury,the rats were killed. The immunohistochemistry skill was employed to detect changes of the expression of PCNA, C-myc and P53 in all rats. Results: On 14th days after angioplasty in medical treatment group, the vessel intima areas were significantly decreased in compared to injury group i the PCNA and C-myc expression of vascular smooth muscle cells were lower, the P53 expression of vascular smooth muscle celis were significantly higher than those of the injury groups. Conclusion: Valsartan possesses inhibitive effects on the proliferation of vascular smooth muscle celis and the expression of C-myc oncogene, but it increases the expression of P53 tumor suppressor gene. By these effects, Valsartan can prevent restenosis after angioplasty.
出处
《心血管康复医学杂志》
CAS
2004年第4期351-353,共3页
Chinese Journal of Cardiovascular Rehabilitation Medicine
