摘要
目的观察严重烧伤应激后糖皮质激素受体(GR)的变化特点以及兴奋性氨基酸谷氨酸的3类受体(代谢型受体、离子型NMDA受体、离子型非NMDA受体)对其表达的调节,以期寻找创伤后GR的可靠调节手段。方法以小鼠背部造成30%总体表面积Ⅲ度烧伤,利用RT-PCR技术,检测烧伤后小鼠海马GR转录水平的变化特点;通过Western-blot方法检测GR蛋白水平的变化特点;选取烧伤后GR表达最明显的时相点,分别检测烧伤前腹腔注射谷氨酸的3种受体拮抗剂[NMDA受体拮抗剂(MK-801)、非NMDA受体拮抗剂(CNQX)、代谢型受体拮抗剂(MCPG)]对伤后GRmRNA水平的影响。结果烧伤各组海马GRmRNA水平明显降低;各组海马GR蛋白水平的表达亦明显降低;烧伤前30min腹腔注射MK-801可显著抑制伤后(2h)GRmRNA水平的下降,而腹腔注射CNQX,MCPG却无此作用。结论严重烧伤可导致海马GR减少,谷氨酸的离子型受体NMDA受体参与了这种变化,提示调控NMDA受体活性可调节创伤后GR的改变。
AIM:To observe the changing characteristics of glucocorticoid receptors (GR) and the adjustment of the three recipients(metabotropic receptors, N methyl D aspartic acid (NMDA) and AMPA kainate (non NMDA) of excitatory amino acids glutacid to the expression in order to search a reliable adjusted function for GR after wound. METHODS: Mice were inflicted with 30%total body surface(TBSA)full thickness burn.Changing characters of GR transcription levels of mice were determined and the changing characters of GR albumen levels were determined with Western blot method.The points whose GR expression were the most transparent after burning were selected to determine the effects of the three antagonist of aminoglutaric acid[NMDA recipient antagonist(MK 801),non NMDA recipient antagonist(CNQX), metabolism recipient antagonist (MCPG)]which were injected intraperitoneally on the GR mRNA level after burn.RESULTS:GRmRNA levels in hippocampi were markedly decreased after burn, and the expressions of GR protein levels in hippocampi were also decreased significantly in all the groups. Intraperitoneal injection of MK 801 30 minutes before burn could restrain the decrease of GR mRNA level, but the injection of CNQX, MCPG did not have the effect. CONCLUSION:Serious burn can result in the decrease of Hippocampal GR,NMDA selective glutamate receptors are involved in these changes,which hints that the NMDA recipient activity can regulate the GR changes after trauma.
出处
《中国临床康复》
CSCD
2004年第23期4765-4767,共3页
Chinese Journal of Clinical Rehabilitation
基金
国家九七三创伤项目01课题(G1999054201)~~
