摘要
基质辅助激光解吸附电离/飞行时间质谱技术(MALDI-TOF MS)是近年来出现的一种精确分子量测定和结构分析的新型平台。以其高灵敏性、高准确度、分析速度快、测量范围宽、分辨率强、样品用量少等优点被广泛应用于检测多肽、蛋白质、核酸、多糖等生物大分子的分子质量和纯度、了解分子间相互作用及翻译后修饰、进行蛋白质和寡核苷酸测序以及高分子聚合物的分子质量分布等。
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ( MALDI-TOF MS) is a newly-developed platform especially suitable for accurate weighing and structural analysis of biomolecules. It is characterized by high sensitivity, high accuracy, inherent simplicity, broad mass detection ranges, high resolution and low sample consumption. This technique is extensively used in verifying sizes and purities of biomolecules, such as peptides, proteins, oligonucleoti-des, and polysaccharides, investigating higher order protein structures (molecule interactions, con-formational differences in allosteric proteins and posttranslational modifications) , identifying sequences of proteins and oligonucleotides and assaying macromolecule polymer distribution ranges.
出处
《医学分子生物学杂志》
CAS
CSCD
2004年第3期190-193,共4页
Journal of Medical Molecular Biology
关键词
基质辅助激光解吸附电离
飞行时间质谱技术
生物芯片
基因
蛋白质
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry ( MAL-DI-TOF MS) , biochip, gene, protein
