摘要
从果园土样中分离筛选获得一株果胶酶产生菌株F_(77),其酶活性为251.5u/ml,该菌经鉴定为泡盛曲霉(Aspergillus awamori Nakazawa),用紫外线诱变选育获得突变株F_(77-101),酶活性为323.1u/ml.F_(77-101)产酶最适培养基为:250ml三角瓶装5g麸皮,0.5g果胶,2％(NH_4)_2SO_4 6ml,产酶最适条件为:pH5.0,25℃,4天.采用超滤——冰冻干燥法提取纯化酶,所得酶制剂的果胶酶活性水平为1293.1u/g(酶),酶性质研究结果表明:酶作用最适pH4.0,温度55℃,热稳定性较好,55℃保温25分钟酶活性尚存60％,EDTA对其有强烈抑制作用。
Based on soil sampling and screening of fungi-producing pectic enzymes, a strain F_(77) of Aspergillus awamori is selected as a most potent organism. Its enzyme activity can reach 251.5u/ml. After F_(77) is mutagenically treated with U. V., a mutant F_(77-101) obtained. Enzyme activity of culture filtrate of F_(77-101) is 323. 1u/ml. The optimum medium consisted of 58 wheat bran, 2g pectin and 6ml of 2% (NH_4)_2SO_4. The optimum culture condition is: 25℃, pH5. 0 and a period of incubation for 4 days. Enzymic preparation is obtained from the culture filtrate by ultrafiltration and freeze-dried, its enzymic activity is 1293. 1u/g. The studies on the properties of enzyme are as foilows: the optimum pH and temperature are pH4.0, 55℃. After incubating at 55℃ for 20 mins, 60% enzyme zctivity remained. EDTANa is a strong inhibetor.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
1993年第4期73-80,共8页
Journal of Fujian Normal University：Natural Science Edition
