摘要
The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination of peptides, identified these metabolites as three peptaibols named Trichokonin Ⅵ,Ⅶ and Ⅷ, and their sequences were confirmed with NMR.
The use of microorganisms for biological purposes has become an effective alternative to control plant pathogens. Trichoderma koningii Smf2 was chosen from eight Trichoderma strains for its thermostatic metabolites with antibiotic activity against Ralstonia solancearum Smith. Exclusion chromatography (LH20) was used twice to partially purify targeted metabolites combined with biological test. LC/ESI-MS, a powerful tool for rapid identification and sequence determination of peptides, identified these metabolites as three peptaibols named Trichokonin Ⅵ,Ⅶ and Ⅷ, and their sequences were confirmed with NMR.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2004年第4期439-439,共1页
Journal of Zhejiang University:Agriculture and Life Sciences
关键词
抗生素
生物防治
隔离
纯化
木霉属
真菌
biological control
peptaibols
ESI-MS
NMR
Trichoderma
Ralstonia solancearum
