摘要
目的:观察甲氧沙林(8-MOP)对体外培养的人毛囊外根鞘无色素黑素细胞黑素合成相关酶的影响。方法:3种浓度(10、50、100μmol/L)的8-MOP作用于体外培养的人毛囊外根鞘无色素黑素细胞,经免疫荧光染色后,采用激光共聚焦显微镜观察不同浓度、不同作用时间的8-MOP对无色素黑素细胞形态的影响,荧光定量分析酪氨酸酶、酪氨酸相关蛋白(TRP)1、TRP2表达量的变化。结果:50、100μmol/L8-MOP作用于人毛囊外根鞘无色素黑素细胞3d能显著促进酪氨酸酶的表达(P<0.01)熏作用7d后TRP1、TRP2的表达也增加(P<0.05)。结论:8-MOP在体外能直接刺激毛囊外根鞘无色素黑素细胞的活化。
Objectives:To study the effects of methoxsalen on the expression of melanogenesis associated enzymes of ame-lanotic melanocytes in outer root sheath of hair.Methods:Three different concentrations of methoxsalen were incubated with amelanotic melanocytes for24h,72h and120h respectively and the expression of tyrosinase,tyrosinase related protein-1and-2(TRP1and TRP2)was measured with laser scanning confocal microscopy.Results:Amelanotic melanocytes treated with50μmol/L,100μmol/L methoxsalen for3days could significantly increase the expression of tyrosinase(P<0.01),and the levels of TRP1and TRP2were also elevated after incubation for7days(P<0.05).Conclusion:methoxsalen could directly stimulate the activation of amelanotic melanocytes.
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2004年第8期471-473,共3页
Journal of Clinical Dermatology
基金
国家自然科学基金资助项目30170861
