摘要
目的 探讨炎症性细胞因子TNF α ,IL 1β和IL 6在沙眼衣原体肺感染中的产生及与机体防御的关系。方法 用沙眼衣原体小鼠肺炎株 (MoPn)通过鼻腔感染小鼠 ,用过氧化物酶连接的鼠抗衣原体脂多糖单抗染色HeLa 2 2 9细胞检测衣原体在肺组织的生长 ;通过测定中性粒细胞髓过氧化物酶 (MPO)检测中性粒细胞在小鼠肺组织中的聚集 ;用RT PCR检测小鼠肺组织炎症性细胞因子mRNA表达。结果 MoPn感染后第 2天 ,肺组织有衣原体生长 ,于感染后第 7天达高峰 ,第 2 1天清除感染的衣原体。感染后第 3天 ,前炎症细胞因子TNF α ,IL 1β和IL 6在小鼠肺组织中的表达明显增高 ,IL 1βmRNA表达于感染第 7天后降低 ,而TNF α和IL 6mRNA的表达至感染后第 14天仍维持较高水平。高水平的TNF α ,IL 1β和IL 6表达出现于中性粒细胞浸润的高峰期。结论 衣原体肺感染诱导前炎症细胞因子TNF α ,IL 1β和IL 6的高表达 ,可能具有中性粒细胞趋化活性 。
Objective To investigate the expressions of proinflammatory cytokines following Chlamydia trachomatis lung infection in mice and its relationship with host immune defence. Methods A murine model of pneumonia induced by intranasal inoculation with Chlamydia trachomatis mouse pneumonitis (MoPn) biovar was used for the study. Chlamydial growth in the lung was assessed by inoculating HeLa 229 cell monolayer with lung homogenates followed by HRP conjugate anti-chlamydial LPS mAb. Neutrophil aggregation in the lung was quantitated by myeloperoxidase (MPO) assay. The mRNA expressions of inflammatory cytokines in the lung were determined by RT-PCR. Results Chlamydial growth in the lung was found at 2 d after infection, and reached its peak at 7 d with subsequent decline in quantity. The infected chlamydia was virtually cleared from the lung at 21 d. The mRNA expressions of TNF-α, IL-1β, and IL-6 were significantly increased in the lung at 3 d after infection, The IL-1β mRNA expression started to decrease at 7 d after infection, but The TNF-α and IL-6 mRNA expressions remained high level until day 14 postinfection. The peaks of TNF-α, IL-1β, and IL-6 mRNA expressions was associated with the peak of neutrophil influx (day 3 postinfection). Conclusion Chlamydia trachomatis can induce high mRNAs expression levels of TNF-α, IL-1β, and IL-6 in the lung, which may contribute to neutrophil chemotaxis and anti-chlamydia immune defence.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2004年第4期274-276,280,共4页
Immunological Journal
