摘要
目的 研究重组人生长激素 (rhGH)在体外对人胃癌细胞生长的影响。方法 实验分组 :对照组、rhGH组、奥沙利铂 (L OHP)组和rhGH +L OHP组 ,利用体外细胞培养、MTT比色技术及流式细胞仪等方法 ,测定不同浓度的rhGH对人胃癌细胞株BGC82 3生长曲线、细胞抑制率、细胞周期和增殖指数 (PI)的影响。结果 rhGH在体外无明显促进BGC82 3细胞分裂增殖 ,rhGH组与对照组、rhGH +L OHP组与L OHP组比较差异均无显著性 (P >0 .0 5 ) ,且生长曲线没有升高 ;rhGH +L OHP组与对照组比较或rhGH +L OHP组与对应的rhGH组配对比较 ,细胞抑制率增加 ,阻滞于G0 ～G1期的细胞数增加 ,S期细胞明显减少 ,PI明显降低 (P <0 .0 1)。rhGH +L OHP组与L OHP组比较 ,细胞抑制率呈现升高趋势 ,而PI呈下降趋势 ,提示rhGH与抗肿瘤药合用可以增强抗肿瘤药对肿瘤细胞的杀伤作用。结论 rhGH在体外无明显促进胃癌细胞的分裂增殖 ,与抗肿瘤药合用可提高抗肿瘤药的疗效。
Objective To study the effects of recombinant humangrowth hormone (rhGH) on growth of human gastric cancer cell in vitro.Methods Four groups were assigned:control group,rhGH group,Oxaliplatin (L-OHP) group and rhGH+L-OHP group.By cell culture,MTT assay and flow cytometry,effects of differenct concentrations of rhGH on cell inhibitory rate,cell cycle and proliferation index (PI) of human gastric cancer line BGC823 were studied.Results In vitro the distinctly accelerated effects of rhGH on multiplication of BGC823 cell line were not found.There was no statistically significant difference between rhGH group and control group or between rhGH+L-OHP group and L-OHP group ( P >0.05).It was not found that the cured line of cells growth rose.Cell inhibitory rate and the cells arrested in G 0-G 1 phase were obviously increased,while the cells in S phase and PI distinctly decreased in rhGH+L-OHP group as compared with control group or rhGH group ( P <0.01).Cell inhibitory rate showed an increase trend but PI showed a decrease trend in rhGH+L-OHP group as compared with L-OHP group.Conclusion In vitro rhGH does not accelerate the multiplication of human gastric cancer cells.Used in combination,it may increase the curative effectivenessof anticancer drugs on cancer cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2004年第5期521-523,共3页
Chinese Journal of Experimental Surgery
