摘要
用分子生物学方法获得了D-氨基酸氧化酶(DAAO)的表达质粒(pPIC3.5k-DAAO),用其电转化巴斯德毕赤酵母GS115获得重组菌,经筛选获得高表达菌株PD27,并对其高密度培养和诱导条件进行了优化。PD27工程菌经250ml摇瓶发酵得到的DAAO活力达到2500～2600IU/L,在14L发酵罐中的表达水平优于其它表达系统。
The expressing plasmid [pPIC3.5K-D-amino acid oxidase(DAAO)]was constructed by methods ofmolecular biology and was transformed into the competent cells of Pichia pastoris GS115. The recombinant strains wereobtained and a high expression recombinant strain PD27 was screened by methanol induction. The conditions for growth andinduction of recombinant strain PD27 were then optimized and DAAO expression level reached 2500～2600IU/L in 250mlflask and 12000IU/L in 14L fermentor which was higher than that found in other expression system.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2004年第7期397-401,共5页
Chinese Journal of Pharmaceuticals
