摘要
目的 构建hsp70 /CD80嵌合DNA真核表达质粒 ,并对其进行鉴定。方法 采用基因工程技术 ,分别以pBR32 2-hsp70和 pcDNA3-CD80质粒为模板 ,经聚合酶链反应 (PCR)扩增出hsp70 /CD80嵌合目的基因 ,然后与 pcDNA3载体进行连接重组 ,构建成hsp70 /CD80嵌合DNA真核表达质粒 ,用限制性内切酶消化、PCR及DNA序列分析等多种方法进行鉴定。结果 hsp70 /CD80嵌合DNA重组真核表达质粒经证实构建成功。 结论 hsp70 /CD80嵌合DNA重组真核表达质粒的成功构建 ,为进一步制备结核病hsp70 /CD80嵌合DNA疫苗奠定了基础。
To construct and identify the eukaryotic expression plasmid of the chimeric DNA hsp70/CD80,the hsp70/CD80 chimeric gene was amplified by PCR,with pBR322 hsp70 and pcDNA3 CD80 used as templates Then the linked recombination was performed with pcDNA3 vector to construct the eukaryotic expression plasmid of the hsp70/CD80 chimeric DNA This plasmid was identified by means of restricted enzyme digestion,PCR and DNA seguence analysis.Results showed that this kind of plasmid was successfully constructed by the mentioned method and the accuracy of this construct was confirmed by a series of molecular biology techniques This method could be used for the preparation of new tuberculosis vaccine
出处
《中国人兽共患病杂志》
CSCD
北大核心
2004年第5期371-374,共4页
Chinese Journal of Zoonoses
基金
国家自然科学基金资助
批准号:3 9870 70 3
四川省重点学科建设项目资助 批准号 :SZD0 42 1