摘要
目的 :观察自体内皮细胞移植在损伤动脉血管再内皮化及抑制新生内膜增生中的作用。方法 :30只健康雄性纯种新西兰兔行双侧髂股动脉球囊损伤 ,一侧于损伤后立即经球囊导管行自体静脉内皮细胞移植 ,另一侧行培养基对照。其中 10只动物分别于术后 4h、4d处死 ,行扫描电镜检查 ;5只动物 ,先将细胞用荧光标记物标记后 ,再移植入体内 ,4d后进行荧光示踪检查 ;5只动物于术后 4d行Evansblue染色 ,观察内皮损伤血管段再内皮化情况 ;其余动物于术后 2 8d处死 ,行病理学分析。结果 :细胞移植术后 4h ,对照组见整个内皮层剥脱 ,暴露出内皮下弹力膜及平滑肌 ,细胞移植组则见部分移植内皮已粘附在内皮剥脱血管壁 ,细胞呈圆形 ,但尚未铺展 ;细胞移植术后 4d ,移植的内皮已变形 ,在内皮剥脱血管壁铺展成单层 ,大量荧光标记内皮细胞被覆在损伤动脉血管内膜 ;对照组损伤血管几乎完全被Evansblue染成蓝色 ,细胞移植组损伤血管则 6 0 %不被Evansblue着染 ;病理学分析发现细胞移植组新生内膜面积、最大动脉内膜厚度均显著少于对照组。结论 :自体内皮细胞能有效地经球囊导管移植到内皮损伤血管段 ,并有减轻新生内膜增生的作用。
AIM: To investigate the feasibility of reendothelialization of the injured arterial wall by autologous endothelial cell transplantation and their influences on neointima proliferation. METHODS: New Zealand white rabbits (n=30) were subjected to bilateral iliofemoral artery balloon injury. Cultured, autologous venous endothelial cells were immediately transplanted into one vessel(transplantation group), whereas the contralateral artery received medium only(control group). Reendothelialization of the injured arterial wall was analysed 4 hours or 4 days after cell transplantation by fluorescent tracing、scanning electron microscope(SEM) and Evans blue staining. Pathology analysis was employed 28 days after cell transplantation to evaluate neointima proliferation. RESULTS: The transplanted endothelial cells had adhered into the aterial wall 4 hours after transplantation and began to attach and spread 4 days later. A number of fluorescent labeling endothelial cells were observed in the endothelial injured arterial wall. The vessels in control group were stained nearly completely by Evans blue, whereas about 60% area was not stained in transplantation group. Pathological examination demostrated that neointimal area and maximal intima thickness in transplantation group significant decreased than those in control. CONCLUSION: Autologus endothelial cells were effectively transplanted into the injured arterial wall by balloon catheter, and it can relieve neointima proliferation in the long time.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2004年第6期919-923,共5页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目 (No .30 2 70 5 6 8)
关键词
细胞移植
内皮
新生内膜增生
Cell transplantation
Endothelium
Neointima proliferation
