摘要
建立了测定牛奶中土霉素残留的RP -HPLC方法。采用草酸甲醇液提取牛奶中的土霉素 ,离心 ,用C18色谱柱 (5 μm ,2 5 0mm× 4 .6mm) ,甲醇 +乙腈 +0 .0 1mol/L草酸溶液 (2 0∶12∶6 8)为流动相 ,流速 1mL/min ,检测波长 36 0nm ,以HPLC外标法测定牛奶中土霉素的残留量。结果 :土霉素峰面积与浓度呈良好的线性关系 ,其线性范围为 5 0～ 15 0 0 μg/L(r =0 .9999) ,平均回收率为 88%。本法简便、准确、快速 ,是一种较好的测定牛奶中土霉素残留的方法。
The method for determining oxytetracycline residues in milk by RP-HPLC was established. Oxytetracycline in milk was extracted by methanol with oxalic acid. After being centrifuged, the extract was assayed by HPLC with C_(18) column(5 μm,250 mm×4.6 mm) and methanol-acetonitrile - 0.01mol/L oxalic acid (20∶12∶68,(V/V)) as the mobile phase at flow rate of 1 mL/min. The detection wavelenght was at 360 nm. The standard curve was linear for oxytetracycline in the range of 50～1 500 μg/L(r=0.999 9,n=6). The average recovery(n=5) was 88%. The method is simple, accurate and rapid.
出处
《中国兽药杂志》
2004年第8期13-14,12,共3页
Chinese Journal of Veterinary Drug
