摘要
目的 研究乙型肝炎病毒 X蛋白 (HBX)对肝癌耐药相关基因 MDRI,MRP1 ,MRP2 3,MRP3,L RP的影响从而探讨 HBX基因影响肝癌多药耐药性状的分子机制。方法 应用脂质体介导技术对人肝癌细胞株 Hep G2瞬时转染 HBX基因 ,然后用逆转录 -聚合酶链反应 (RT- PCR)和 Westernblot技术分别检测转染后多药耐药相关基因和蛋白水平的表达情况。再使用 MTT检测阿霉素及丝裂霉素诱导后细胞存活率的变化。结果 转染前后相比 ,转染 HBX基因的 Hep G2细胞多药耐药相关基因和蛋白表达水平比转染前均明显上调 (P<0 .0 5 )转染后 PRP1基因表达量与转染前比为 3.1 1 ,PRP2为 1 .6 9,L RP3为 1 .4 6 ,MDR1为 3.6 4 ,L RP为 1 .90。 Westernblot显示转染后 L DR1蛋白水平上升 1 .1 0倍 ,MRP1蛋白上升 1 .2 8倍 ,L RP蛋白上升 1 .0 8倍。MTT结果显示阿霉素和丝裂霉素 IC50 转染组明显高于对照组。结论 乙型肝炎病毒
Objective To study the influence of HBX protein on the expression of hepatic carcinoma multidrug resis tance associatedgenes:MDR1,MRP1,MRP2,MRP3,LRP.Methods Liposome carrying HBX gene was used to reansfect HepG2 cell line.The expression of the multidrug resistance associated genes and proteins were measured by RT PCR and Western blot.MTT was used to check the viability of cella exposed to ADM and MMC.Result Comparing with control group the levels of multidrug resistance associated genes and proteins of the transfected HepG2 cell were signigicantly higher( P <0.05).The similar change was witnessed in the protien level which confirmed by Western blot.The result of MTT also revealed that theviability of transfected cell is higher than that of the control group.Conlusion These data demonstrated that HBX protein may play a causative role in the development of MDR of HCC.
出处
《肝胆外科杂志》
2004年第3期214-216,共3页
Journal of Hepatobiliary Surgery
关键词
HBX基因
多药耐药
MTT转染
HBX gene
Multidrug resistance(MDR)
MTT
Transfection
