摘要
以成纤维细胞为载体,建立了IL-6基因疗法的实验模型并研究了其免疫调节作用。将人IL-6cDNA插入到携有新霉素抗性基因的表达质粒BCMGNeo上,用磷酸钙共沉淀法将BCMGNeo-IL-6转染入成纤维细胞NIH3T3中,用G_(418)抗性筛选和有限稀释法,挑选到一株高分泌IL-6的成纤维细胞克隆(T6.6)。将T6.6移植到小鼠腹腔内,12h后即可从血清中枪测出IL-6,至96h,血清中IL-6仍维持在较高水甲。T6.6体内移植后能显著增强淋巴细胞增殖反应和IL-2产生水平。表明成纤维细胞介导的IL-6基因疗法能有效地增强免疫功能,可望为肿瘤治疗开辟一条新途径。
Interleukin-6 (IL-6) is a pleiotropic cytokine which has antitumor activity. In the present study, a model for fibroblasts-mediated IL-6 gene therapy and its immune regulation are described. Human IL-6 cDNA was inserted into plasmid vector BCMGNeo containing a neomycin resistance gene. BCMGNeo-IL-6 was transferred into NIH3T3 fibroblasts by calcium phosphate coprecipitation method. A fibroblast clone (T6.6) secreting 1L-6 at highest level was selected by G418 resistance selection and limiting dilution. When T6.6 was implanted i.p. into mice, IL-6 could be detected in serum after 12 h. Even after 96 h, serum IL-6 still maintained at high level. Lymphocyte proliferation and IL-2 production could be enhanced significantly after in vivo implantation of T6.6. These results demonstrate that fibroblasts -mediated IL-6 gene therapy could augment immune function efficiently and outline a novel strategy for cancer treatment.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1993年第3期210-215,共6页
Academic Journal of Second Military Medical University
关键词
白细胞介素6
成纤维细胞
基因治疗
interleukin-6
fibroblasts
gene therapy
interleukin-2
mice
