摘要
目的:建立救脑滴丸中人参皂苷Rg1、Rb1的含量测定方法。方法;采用C18(10μm,250mm×4.6mm),人参皂苷Rg1用乙腈-0.05％磷酸(1 :3),人参皂苷Rb1用乙腈-0.1％磷酸(33:67),流速:1.0ml/min,检测波长:203nm。结果:人参皂苷Rg1、Rb1在0.508-5.08μg线性关系良好,平均回收率人参皂苷Rg1为101.59％,RSD为2.57％,人参皂苷Rb1为101.28％,RSD为2.50％。结论:本法操作简便,结果稳定,重现性好,可作为质量控制方法。
Objective:to establish the HPLC method of content measuration of ginsenoside Rg1 and Rb1 in JiuNao Drop Pills. Method:C18 column(10μm,250mm×4. 6mm)was used with a mobile phase of acetonitrile-0. 05% H3PO4(1 : 3,Ⅴ : Ⅴ)in measuration of ginsenoside Rg1 and acetonitrile-0. 05% H3PO4(1 : 3,Ⅴ : Ⅴ)in measuration of ginsenoside Rb1 and with a speed of 1.0ml/min. The wavelength of the detector was set at 203nm. Result:The linear ranges of the ginsenoside Rg1 and Rb1 are both 0. 508-5. 08. The average recovery rates are 101. 59% and 101. 28%. The RSD are 2. 57% and 2. 50% respectively. Conclusion:The method is simple,accurate,with a good reproducibility,and it can be applied to the quality control of ginsenoside Rg1 and Rb1.
出处
《中国药品标准》
CAS
2004年第3期51-54,共4页
Drug Standards of China
