摘要
超声波破碎黑曲霉细胞提取游离果糖转移酶,然后选择D201大孔阴离子交换树脂为载体,通过先吸附后交联的方法固定果糖转移酶.优化的固定化条件:加酶量为每克湿树脂400U,吸附pH值为5.0～5.5,吸附温度为30℃,吸附时间为8h,交联剂戊二醛(终)质量浓度为0.01～0.05g/dL,交联时间为8h,交联温度为1～4℃.固定化酶活最高回收率为30.2%.
Fructosyltransferase was extracted from the cells of Aspergillus niger with ultrasonic treatment, and then was immobilized through adsorbtion and crosslinking. The carrier was D201 macroporous anion-exchange resin. The optimum conditions for the immobilization were as follows: 400 U of enzyme per g wet resin, pH 5.0～5.5, 30 ℃, and 8 hours, respectively. The crosslinking temperature and time were 1～4 ℃ and 8 h respectively, and the concentration of the crosslinking agent (glutaraldehyde) was 0.01～0.05 g/dL.The maximum yield of immobilized enzyme was 30.2 %.
出处
《无锡轻工大学学报（食品与生物技术）》
CSCD
北大核心
2004年第4期60-64,共5页
Journal of Wuxi University of Light Industry
关键词
低聚果糖
果糖转移酶
固定化
阴离子交换树脂
fructooligosaccharides
fructosyltransferase
immobilization
anion-exchange resin
