摘要
目的:探讨不同时段超深低温保存对骨组织生物力学性能的影响。为吻合血管同种异体骨关节保存移植提供力学依据.方法:采用两步冷冻法,以15％DMSO为低温保护剂,用-55℃对SD大鼠股骨进行冷冻预处理。液氮中保存18天.180天,测试首用40℃水浴复温.每组股骨各5段,分别与对照组进行抗压和抗折实验.结果:骨压缩强度极限(MPa),对照组.18天、180天组分别为77.26±11.80、76.02±15.00和61.90±12.20:股骨弯曲最大挠度,对照组、18天.180天组分别为44.60±10.54、44.20±9.50和20.01±7.58.结论:随着超深低温保存时间的延长,SD大.鼠股骨抗拉的延伸事下降,表明骨的脆性增加,建议尽量缩短超深低温保存时间.
Objective: To investigate the biomechanics effect of bone tissue with ultra cryopreservation in different phase and to provide biomechanics basis for allogeneic transposition of bone and joint with vessel. Methods: The study using femur of SD rats was divided into experimental group and control group. The femur of SD rat in experimental group underwent -55℃ preliminary cold treatment and then preserved in liquid nitrogen for 18, 180 days respectively. Each group with 5 femur was given restitution temperature with 40℃ water before experiment and then underwent antipressure and antifracture test Results: The limiting value of bone pressure strength in control group was 77.26±11.80, 18 day's group was 76.±15.00 and 180 day's group was 6t.90±12.20. The maximal deflection of the femur arcuation in control group was 44.60± 10.54, 18 day's group was 44.20±9.50 and 180 day's group was 20.0± 17.58. Conclusion: The cardinal biomechanics value of femur in the SD rat no significant difference was observed in each experimental group and that of control. However, there was statistical difference in between control group and 180 day's group, 18 day's and 180 day's group, retrospectively. The prolonged rate of bone anti-extension reduced and the crispness of bone increased following time of cryopreservation prolonged. The probable shortening the time of cryopreservation should be performed.
出处
《伤残医学杂志》
2004年第2期4-6,共3页
Medical Journal of Trauma and Disability
关键词
超深低温
SD
大鼠
骨组织
生物力学
修复材料
Ultra-cryopreservation
Homograft
Bone tissue
Bone transplantation
Biomechanics
Different phase
