摘要
背景与目的BP1(beta-protein1)基因是新近发现的转录因子,定位于染色体17q21-22,属同源异型盒基因DLX家族,在急性髓系白血病和急性T淋巴细胞白血病中均呈过度表达。本研究通过观察BP1mRNA在乳腺癌中的表达情况,分析并探讨其与乳腺癌临床病理特征的关系。方法以β-actin基因作为参照,应用逆转录聚合酶链反应(RT-PCR)技术,检测82例乳腺肿瘤组织、12例近端肿瘤旁组织和10例远端癌旁组织中BP1mRNA的表达。结果82例肿瘤组织中有53例BP1阳性(64.63%),而在近端癌旁组和远端癌旁组中则表达很弱(16.67%)或不表达(0%),差异具有统计学意义(P<0.05)。BP1基因的过表达与组织学分级有关(P<0.05),而与肿瘤大小、有无淋巴结转移、病理类型、肿瘤家族史、初潮年龄、初产年龄、怀孕次数、绝经状况、雌激素受体及孕酮受体状态无关。结论BP1基因在部分乳腺癌中上调,其表达与肿瘤组织学分级有关。
BACKGROUND & OBJECTIVE: Beta protein 1 (BP1) gene, a novel member of DLX homeobox gene family, is located in 17q21 22 region and overexpressed in both acute myeloid leukemia and acute T cell lymphocytic leukemia. However, the reports on the function of BP1 in solid tumors are rare. The study was designed to determine the expression of BP1 gene in breast cancer and to analyze its relationship with various clinicopathological factors. METHODS: With β actin gene as a reference, BP1 mRNA expression was detected in 82 breast cancer tissues and 12 near adjacent tissues and 10 far adjacent tissues using reverse transcription polymerase chain reaction (RT PCR). RESULTS: The expression rates of BP1 in near and far adjacent tissues were 16.67%(2/12) and 0% (0/10), respectively; while the expression rate in breast cancer group was 64.63% (53/82). There were statistically significant differences among the three groups (P< 0.05). Overexpression of BP1 in breast cancer was correlated with histological grade (P< 0.05). Nevertheless, no correlation was found between BP1 expression and other clinicopathological factors, including tumor size, lymph nodal metastasis, family history, pathological type, menarcheal age, primiparous age, number of pregnancy, menopausal status, ER status and PR status (P >0.05). CONCLUSION: BP1 gene is of upregulated expression in breast tumors and could be regarded as a new and vital biomarker in breast cancer research.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2004年第7期855-859,共5页
Chinese Journal of Cancer
关键词
乳腺肿瘤
BP1基因
同源异型盒
基因表达
逆转录聚合酶链反应
诊断
Breast neoplasms
BP1 gene
Homeobox
Gene expression
Reverse transcription polymerase chain reaction
Diagnosis
