摘要
将鼠疫耶尔森氏菌6MD质粒转入假结核耶尔森氏菌中,该质粒决定了杂交菌株中PstI(45kD),Pla蛋白(37kD,35kD)的合成。杂交株膜蛋白制剂的十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)图形与其亲本株比较,外膜蛋白1(Yop1)确实发生了降解,但这种作用较弱。值得注意的是在与亲本株菌Yop1蛋白完全相同的迁移位置上仍有相当含量的这种蛋白没有发生降解。这一结果与国外同类研究的结论有显著差异。本文就这种现象可能产生的影响进行了分析。
6MD plasmid in Yersinia pestis was transformed into the cells of Yersinia pseudotuberculosis. The plasmid determined the syntheses of PstI(45 kD) and Pla proteins (37, 35kD) in the hybrid strain. A comparision of the pattern of sodium dodecyl sulfatepolyacrylamide gel electrophorisis (SDS-PAGE) between the membrane preparation of the hybrid strain and that of its parent showed that the degradation of Yop1 in the hybrid did occur, but this effect was weak. It is important that quite much quantity of this protein which remained in identical migratory position with Yopl in the parient strain could not degrase. The results of the present study differed from those in some foreign reports.
出处
《地方病通报》
1993年第1期45-48,共4页
Endemic Diseases Bulletin
基金
国家自然科学基金资助项目
关键词
质粒
外膜蛋白
降解
鼠疫杆菌
Plasmid
Yersinia pestis
Yersinia pseudotubberculosis
SDS-PAGE
Hybrid strain
Degradation
Yop1
Transformation
Reversion
