摘要
【目的】探讨提高昆虫病原线虫冷冻存活能力的方法,以改进昆虫病原线虫储存技术。【方法】以我国高寒地区分离、筛选出的高致病力昆虫病原线虫Heterorhabditis bacteriophora-HBN(HBN,45°45′N)和从美国引进的同种不同品系昆虫病原线虫H.bacteriophora-NJ(NJ,40°13′N)为研究对象,在实验室条件下研究了不同预处理方式对2个品系线虫冷冻存活率的影响。【结果】HBN经过25℃7d、10℃7d、4℃7d、25℃3d10℃3d和25℃3d10℃3d4℃3d的预处理后,于-4℃冷冻5d时的存活率为86.1%~95.2%,显著高于直接冷冻法(25℃0d)的存活率17.7%,而NJ经25℃3d10℃3d4℃3d的预处理后,于-4℃冷冻5d时的存活率为83.0%,显著高于其他预处理(2.2%~58.5%)。【结论】适当的预处理可提高2个品系线虫的冷冻存活率,但生活在不同地理纬度的同种线虫(HBN和NJ)适于不同的驯化方式。
【Objective】In order to improve storage technology of entomopathogenic nematodes(EPNs),the methods to increase freezing survival of EPNs were explored.【Method】Two strong virulence strains of EPNs,Heterorhabditis bacteriophora-HBN(HBN)with cold tolerance isolated from Harbin(China,45°45′N)and H.bacteriophora-NJ(NJ)from New Jersey(USA,40°13′N),were used in this study.The survival rates of HBN and NJ at-4 ℃for 5days after different pretreatment methods were compared.【Result】The freezing survival rates of HBN pretreated at 25 ℃for 7d,10 ℃for 7d,4 ℃for 7d,25 ℃for 3d then 10 ℃for 3d,and 25 ℃for 3dand 10 ℃for 3dbefore 4 ℃for 3dwere 86.1%to 95.2%,which were significantly higher than that of 25℃for 0d(17.7%).The highest freezing survival rate of 83.0%,was obtained for NJ when pretreated at 25℃for 3dand 10℃for 3dbefore 4℃for 3d,which was significantly higher than those of other pretreatment methods(2.2%to 58.5%).【Conclusion】Freezing survival rates of HBN and NJ could be increased by appropriate pretreamtents.But the best pretreatment methods for the same species(HBN and NJ)isolated from different geographical latitudes were different.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2015年第2期198-202,共5页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家科技计划农业成果转化项目(2007GB24910482)
国家自然科学基金项目(31000205)
黑龙江省青年科学基金项目(QC2010070)
哈尔滨市科技创新人才项目(2009RFQYN092)
关键词
昆虫病原线虫
预处理
低温驯化
冷冻储存
存活率
entomopathogenic nematode
preprocessing
cold acclimation
freezing storage
survival rate
