摘要
【目的】研究安徽地区猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)ORF3基因序列特征。【方法】于2012-2013年,从安徽省合肥、肥东、肥西、滁州、亳州、阜阳、马鞍山、蚌埠、宿州、巢湖、六安、淮南、宣城和淮北地区的19个不同猪场采集93份病料,采用RT-PCR方法对PEDV ORF3基因进行扩增,测序后进行序列分析,并与韩国、欧洲及中国其他省份PEDV毒株进行同源性比较,并构建遗传进化树。【结果】在93份病料中,自20份病料中获得的PEDVORF3基因开放阅读框全长为810bp,编码224个氨基酸,较多的基因位点发生了突变,没有缺失和插入。安徽省PEDV毒株之间的同源性为96.7%~100.0%,与欧洲株同源性为94.9%~97.0%,与韩国株同源性分别为96.3%~98.7%,与中国其他省份分离株的同源性为95.9%~100.0%。【结论】安徽地区PEDV ORF3基因与韩国株亲缘关系较近,而与欧洲株亲缘关系较远。
【Objective】This study aimed to investigate sequence features of ORF3 gene of porcine epidemic diarrhea virus(PEDV)in Anhui province.【Method】A total of 93 samples were collected from 19 different farms in Hefei,Bozhou,Fuyang,Maanshan,Bengbu,Suzhou,Caohu,Lu'an,Huainan,Xuncheng,and Huaibei in Anhui during 2012-2013.RT-PCR method was used for gene amplification and nucleotide sequences were analyzed after being sequenced.The results were compared with strains from other areas and phylogenetic tree was constructed.【Result】20 ORF3 genes out of 93 samples from Anhui had the open reading frame of 810 bp,encoding 224 amino acids.A large number of mutations occurred.The homology of ORF3 gene in Anhui PEDV were 96.7%-100.0%.The homology of ORF3 gene compared with European,Korea and other China strains were 94.9%-97.0%,96.3%-98.7% and 95.9%-100.0%,respectively.【Conclusion】ORF3genes of Anhui isolates were genetically close to PEDV Korea strains while distantly related to European strains.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2015年第2期1-6,共6页
Journal of Northwest A&F University(Natural Science Edition)
基金
现代农业产业技术体系建设专项资金资助项目(CARS-36-生猪)
安徽省生猪产业技术体系资金资助项目
关键词
猪流行性腹泻病毒
ORF3基因
基因分析
安徽
pig's porcine epidemic diarrhea virus
ORF3gene
genetic analysis
Ahhui Province
