摘要
目的研究老年人淋巴细胞亚群、脂筏标志分子单唾液酸四己糖神经节苷脂(GM1)和细胞活化与血清胆固醇水平的相关性,探讨高胆固醇水平调节淋巴细胞功能的分子机制以及与动脉粥样硬化的关系。方法样本采用在时间上随机抽样的方法选取2012年8月至2014年9月在天津医科大学总医院体检者60例,年龄60~80岁,其中女30例,男30例。采用抗体染色、流式细胞仪检测外周血中总B淋巴细胞(CD19+)、记忆B淋巴细胞(CD19+CD27+)、初始B淋巴细胞(CD19+CD27-)、总T淋巴细胞(CD3+)、CD3+CD4+和CD3+CD8+T淋巴细胞亚群比例以及神经节苷脂GM1水平;采用细胞内染色方法检测活化细胞内磷酸化Stat3的水平,并与胆固醇水平和动脉粥样硬化的发生进行相关性分析。结果 CD19+B淋巴细胞比例与低密度脂蛋白胆固醇(LDLC)水平、CD19+CD27+B淋巴细胞比例与极低密度脂蛋白胆固醇(VLDLC)水平呈显著正相关(P<0.05);CD3+T淋巴细胞、CD3+CD4+和CD3+CD8+T淋巴细胞比例与胆固醇水平无相关性(P>0.05);记忆B淋巴细胞GM1水平与总胆固醇(TC)和VLDLC、CD3+CD8+T淋巴细胞GM1水平与高密度脂蛋白胆固醇(HDLC)均呈显著正相关(P<0.05)。与正常胆固醇组比较,在BCR抗体刺激后,高胆固醇组记忆B淋巴细胞中磷酸化Stat3水平显著升高(P<0.01)。结论高胆固醇水平是老年人动脉粥样硬化发生的重要危险因素,同时高胆固醇水平与B淋巴细胞比例、脂筏GM1水平、Stat3的活化以及CD3+CD8+T淋巴细胞GM1水平的相关性,提示其可能调节淋巴细胞增殖、活化、炎症因子的产生,从而促进动脉粥样硬化的发生和发展。
Aim To study the correlations between the proportion and GM1 levels of lymphocyte subsets as well as cell activation and serum cholesterol levels and to discuss how high levels of cholesterol regulate lymphocyte functions and the relations to atherosclerosis. Methods A total of sixty subjects were randomly enrolled in this study during their regular physical examination with thirty females and thirty males,aged between 60 and 80 years. The proportions of peripheral blood total B lymphocytes( CD19+),naive B cells( CD19+CD27-),memory B cells( CD19+CD27+),total T lymphocytes( CD3+),CD3+CD4+and CD3+CD8+T cells and their expression of GM1 were determined by antibody staining and flow cytometry. Phosphorylated Stat3 was detected by intracellular staining. The results were analyzed with respect of their correlations with serum cholesterol levels and the occurrence of arthrosclerosis. Results The proportion of CD19+total B cells and low density lipoprotein cholesterol( LDLC) levels as well as the proportion of CD19+CD27+memory B cells and very low density lipoprotein cholesterol( VLDLC) levels showed significantly positively correlations( P < 0. 05). We have not found any significant correlations between T cell subsets and cholesterol levels( P > 0.05). The GM1 levels of memory B cells were positively correlated with both total cholesterol( TC) levels and VLDLC levels( P < 0. 05). The GM1 levels of CD3+CD8+cells were positively correlated with high density lipoprotein cholesterol( HDLC) levels( P < 0. 05). BCR stimulation induced activation of Stat3. There was significant increase in the levels of Stat3 phosphorylation in high-cholesterol group compared with low-cholesterol group( P < 0. 01). Conclusions High cholesterol level is a major risk factor for atherosclerosis in the elderly. The correlations between the high cholesterol levels and the proportion,GM1 levels of lymphocyte subsets and Stat3 activation suggested high cholesterol levels may regulate lymphocyte proliferation,activation,proinflammatory cytokine produc
出处
《中国动脉硬化杂志》
CAS
北大核心
2015年第1期49-53,共5页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金资助项目(81070271)
