摘要
为了研究重组牛抵抗素对小鼠血液中血糖和胰岛素含量的影响,选取体质量相似的4周龄wistar大鼠20只,随机分成4组,分别在禁食2d和自由进食的情况下注射PBS缓冲液和重组牛抵抗素,12h1次,连续4次。随后在0、15、30、60、120、180min分别尾静脉采血,检测血糖和胰岛素含量。试验结果表明重组牛抵抗素不论对自由进食或禁食2d大鼠的血糖浓度和胰岛素水平均具明显提高作用,说明重组牛抵抗素具有明显的生物学活性。
In order to study the effect of recombinant bovine resistin on glucose and insulin in blood of rats,20 wistar rats were randomly divided into 4groups,each group was given a free choice feeding or fasted for 2d,then injected with PBS and recombinant bovine resistin.at an interval of12 h,continuous 4times.Then blood samples were collected at 15,30,60,120,180 min after the first sampling and tested for glucose and insulin blood.The results showed that recombinant bovine resistin could obviously increase glucose and insulin levels in blood of both fasting rats and free-feeding rats,which confirmed the biological activity of recombinant bovine resistin.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2015年第6期949-952,共4页
Chinese Journal of Veterinary Science
基金
四川省科技支撑计划资助项目(2013NZ0032)
