摘要
AIM: To discuss the effects of different concentrations of tetramethylpyrazine(TMP), an active constituent of Chinese herb, on damaged Shandong human corneal epithelial cell(SDHCEC) induced by hydrogen peroxide.METHODS: We detected the combined effects of TMP with concentrations ranging from 4 mg/m L to 0.03 mg/m L and 800 μM hydrogen peroxide on SDHCEC. The methyl thiazolyl tetrazolium(MTT) assay was processed at 3, 6and 12 h separately while the detection of cell apoptosis at 6h only by flow cytometry.RESULTS: The viability of SDHCEC with 0.5 mg/m L,0.25 mg/m L, 0.125 mg/m L and 0.06 mg/m L TMP joint with800 μM hydrogen peroxide at 3h and 6h was significantly higher than that with 800 μM hydrogen peroxide only, P 【0.05. However, except 0.25 mg/m L, TMP with other concentrations joint with 800 μM hydrogen peroxide at12 h could not significantly inhibit decreased SDHCEC viability induced by 800 μM hydrogen peroxide. At 12 h,TMP of 0.5 mg/m L, 0.25 mg/m L, 0.125 mg/m L and 0.06 mg/m L could significantly inhibit SDHCEC early apoptosis induced by 800 μM hydrogen peroxide, most remarkable at 0.25 mg/m L TMP, P 【0.05.CONCLUSION: Our results suggested that hydrogen peroxide can induce apoptosis related damage to SDHCEC. TMP can protect SDHCEC from the damage,and the protective effects may be associated with its anti-apoptosis mechanism.
AIM: To discuss the effects of different concentrations of tetramethylpyrazine(TMP), an active constituent of Chinese herb, on damaged Shandong human corneal epithelial cell(SDHCEC) induced by hydrogen peroxide.METHODS: We detected the combined effects of TMP with concentrations ranging from 4 mg/m L to 0.03 mg/m L and 800 μM hydrogen peroxide on SDHCEC. The methyl thiazolyl tetrazolium(MTT) assay was processed at 3, 6and 12 h separately while the detection of cell apoptosis at 6h only by flow cytometry.RESULTS: The viability of SDHCEC with 0.5 mg/m L,0.25 mg/m L, 0.125 mg/m L and 0.06 mg/m L TMP joint with800 μM hydrogen peroxide at 3h and 6h was significantly higher than that with 800 μM hydrogen peroxide only, P <0.05. However, except 0.25 mg/m L, TMP with other concentrations joint with 800 μM hydrogen peroxide at12 h could not significantly inhibit decreased SDHCEC viability induced by 800 μM hydrogen peroxide. At 12 h,TMP of 0.5 mg/m L, 0.25 mg/m L, 0.125 mg/m L and 0.06 mg/m L could significantly inhibit SDHCEC early apoptosis induced by 800 μM hydrogen peroxide, most remarkable at 0.25 mg/m L TMP, P <0.05.CONCLUSION: Our results suggested that hydrogen peroxide can induce apoptosis related damage to SDHCEC. TMP can protect SDHCEC from the damage,and the protective effects may be associated with its anti-apoptosis mechanism.
基金
Supported by Guangdong Administration of Traditional Chinese Medicine (No.2007095)
