摘要
利用玉米秸秆水解液为基质,采用海藻酸钠包埋方法固定光合菌群,进行固定化光合细菌发酵产氢研究,考察了菌液包埋量、颗粒粒径、海藻酸钠浓度、CaCl2浓度和光照强度的影响。结果表明,当菌液包埋量为4mL,颗粒粒径为2.5mm,海藻酸钠浓度为1.5%,CaCl2浓度为10%,光照强度为7000lux,效果较好,培养72h后,总产氢量最高可达491mL H2/L-培养基,是相似条件下游离态光合菌群发酵产氢的1.2倍,为固定化光合细菌利用玉米秸秆发酵产氢研究提供参考。
Photosynthetic bacteria were fixed with sodium alginate by embedding method based on cornstalk hydrolysate as substrate,to investigate hydrogen produced by bacterial fermentation.The effect of the amount of bacterium suspension embedded,particle size,sodium alginate concentration,CaCl2concentration and illumination intensity were investigated.It indicated that hydrogen production was better when the amount of bacterium suspension embedded was 4mL,particle size was 2.5mm,concentration of sodium alginate was 1.5%,concentration of CaCl2was 10%,and illumination intensity was 7000lux.After cultivating for 72h,the maximum hydrogen production was up to 491 mL H2/L-culture medium,being 1.2 times as that of free photosynthetic bacteria under similar conditions.It will provide a theoretical basis for hydrogen production of immobilized photosynthetic bacteria using corn stalk.
出处
《中国酿造》
CAS
2013年第S1期51-53,共3页
China Brewing
基金
国家高技术研究发展(863)计划项目(2013AA050702)
关键词
固定化
光合菌群
玉米秸秆
产氢
immobilization
photosynthetic bacteria group
corn stalk
hydrogen production
