摘要
目的采用HPLC测定龙血竭脂质纳米粒中龙血素A的含量并计算其包封率。方法采用DiamonsilTMC18(200 mm×4.6 mm,5μm)色谱柱,以乙腈-1%冰醋酸水溶液(38∶62)为流动相,流速为1.3 mL.min 1,检测波长为260 nm,柱温为40℃,测定样品中龙血素A的含量并计算其包封率。结果龙血素A于0.135～5.4μg.mL 1内有良好线性关系,Y=33.694X+1.589 8(r=0.999 9)。低、中、高3个浓度的日内RSD分别为2.70%,2.36%,1.48%;日间RSD分别为2.70%,2.61%,1.97%,该方法的平均回收率为99.2%,RSD为3.9%,平均包封率为89.59%。结论本方法准确、简便、重复性好,可用于龙血竭脂质纳米粒中龙血素A含量的测定。
OBJECTIVE To establish an HPLC method for determination of loureirin A content in solid lipid nanoparticles(SLN) of Resina Draconis.METHODS A DiamonsilTM C18(200 mm×4.6 mm,5 μm) column was used for the determination of loureirin A with mobile phase of acetonitrile-1% acetic acid solution(38∶62).The flow rate was 1.3 mL·min 1,the detection wavelength was set at 260 nm,and the column temperature was 40 ℃.RESULTS The linear range of 1oureirin A was 0.135 5.4 μg·mL 1,Y=33.694X+1.589 8(r=0.999 9).The RSDs of within-day were 2.70%,2.36%,1.48%,and the RSDs of between-day were 2.70%,2.61%,1.97% in the high-,middle-,and low-dose Resina Draconis loaded SLN.The average recovery was 99.2%(RSD 3.9%),and the average drug encapsulation efficiency was 89.59%.CONCLUSION The method is simple and reliable for the determination of loureirin A in Resina Draconis loaded SLN.
出处
《中国现代应用药学》
CAS
CSCD
2013年第1期69-72,共4页
Chinese Journal of Modern Applied Pharmacy
