摘要
目的探讨短时常温(1h,37℃)条件下,非去极化心脏停搏液(NDP)对猪冠脉内皮源性超极化因子(EDHF)介导的血管舒张功能的影响。方法根据保存液的不同,将血管环分成四组,分别为对照(CON)组、St.Thomas(ST)组、HTK组、NDP组。采用器官槽法,将血管环分别置于Krebs-Hensleit(KH)液、ST液、HTK液、NDP液中,37℃条件下保存1 h,加入一氧化氮合酶阻滞剂N-硝基-L-精氨酸(L-NNA)和环加氧酶阻滞剂(Indomethacin),记录由前列腺素F2α(U46619)及缓激肽(Bradykinin)引起的血管收缩和舒张反应。结果 NDP组中EDHF介导的内皮依赖性血管舒张百分比最大,为(74.79±6.40)%,其余依次为HTK组(51.32±21.92)%、CON组(24.03±8.01)%、ST组(16.20±6.39)%。结论短时常温(1 h,37℃)条件下,NDP液对冠脉内皮的舒张功能具有保护作用,且优于HTK组、CON组和ST组。
Objective The present study was designed to evaluate the protective effect of non-depolarizing solution(NDP) on the endothelium-derived hyperpolarizing factor(EDHF)-mediated relaxation of porcine coronary arteries after rings were incubated with NDP solution at 37℃ for 1 hour.Methods Due to different preservation solutions used in different groups,the rings were divided into four groups:control(CON) group,St.Thomas(ST) group,Histidine-Tryptophan-Ketoglutarate(HTK) group and NDP group.Rings from porcine coronary arteries were studied in the organ chamber.After equilibration,the rings were incubated with Krebs-Helenseit solution in CON group,St.Thomas solution in ST group,HTK solution in HTK group,non-depolarizing solution in NDP group at 37℃ for 1hour.Then Pre-contraction tone induced by prostaglandin F2α(U46619) and endothelium-dependent relaxation tone induced by bradykinin was measured in the presence of indomethacin and L-NNA.Results After incubation with different solutions at 37℃ for 1hour,the EDHF-mediated relaxation induced by bradykinin was much higher in the NDP group 74.79%±6.40% than in the HTK group 51.32%±21.92%,CON group 24.03%±8.01% and ST group 16.20%±6.39%.Conclusion NDP solution provides superior endothelium protective effect compared with ST and HTK solution under normal temperature.
出处
《中国体外循环杂志》
2013年第4期252-256,共5页
Chinese Journal of Extracorporeal Circulation
基金
国家自然科学基金资助项目(30972866)
北京市自然科学基金资助项目(7102131)
关键词
血管环
保存液
内皮源性超极化因子
Vascular rings
Preservation solutions
Endothelium-derived hyperpolarizing factor
