摘要
目的研究miR-106b对宫颈癌SiHa细胞转移侵袭功能的影响及作用机制。方法 SiHa细胞转染miR-106b inhibitors和mimics,实时定量PCR检测转染效率,Transwell及划痕法检测细胞运动侵袭功能,分析E-cad-herin、PTEN、p-AKT(Ser473)蛋白表达情况。结果 miR-106b inhibitors和mimics改变SiHa细胞中miR-106b表达。转染miR-106b inhibitors后,细胞迁移数、移动距离减少(P<0.05);PTEN及E-cadherin表达增强,p-AKT表达降低。转染miR-106b mimics后,细胞迁移数、移动距离增加(P<0.05);PTEN及E-cadherin表达降低,p-AKT表达增强。结论 miR-106b可能通过E-cadherin/AKT、PTEN/AKT信号通路影响SiHa细胞运动侵袭功能。
Objective To explore the effect of miR-106b on migration and invasiveness of cervical cancer cells SiHa and its possible mechanism.Methods miR-106b inhibitors and mimics were transiently transfected into SiHa cells,and the expression of miR-106b was detected by real-time PCR.Then,the function of migration and invasion of cells and the expression level of E-cadherin,PTEN and p-AKT were detected by Transwell,wound-healing and Western blotting assay.Results The expression of miR-106b was significantly altered by the miR-106b inhibitors and mimics in SiHa cells.Compared with negative control cells,knockdown of miR -106b expression dramatically decreased the number of cell invasion and the cell migration function (P<0.05).The expression of E-cadherin and PTEN were enhanced,but the p-AKT level was inhibited.On the contrary,compared with negative control cells,over expression of miR-106b significantly increased the cell invasion number and the cell migration function ( P<0.05).The expression of E-cadherin and PTEN were decreased,but the p-AKT level was enhanced.Conclusion The function of SiHa cells migration and invasion may be modulated by miR-106b through the signal pathway of E-cadherin/AKT and PTEN/AKT.
出处
《中国当代医药》
2013年第24期9-11,共3页
China Modern Medicine
基金
国家自然科学基金(81272859)
郑州大学第一附属医院青年创新基金
