摘要
目的分析MicroRNAs在胰腺癌早期诊断中应用的临床价值。方法3种胰腺癌细胞系PANC-1、PaCa-2、AsPC-1和正常胰腺细胞以及胰腺癌患者100例和正常志愿者100例血浆,采用Real-time PCR检测mir-155、mir-196a、mir-181a、mir-181b的表达量,并进行统计分析;分别取胰腺癌组织100例,正常胰腺胰腺组织100例,采用免疫组化从组织水平检测mir-155、mir-196a、mir-181a、mir-181b的表达量,并进行统计分析。结果Real-time PCR检测结果显示,与正常胰腺细胞相比较,PANC-1、PaCa-2、AsPC-1 3种胰腺癌细胞中mir-155、mir-196a的表达量均明显增高(P<0.05);与健康志愿者血浆相比,胰腺癌患者血浆中rnir-155、mir-196a、mir-181a、mir-181b的表达明显增高(P<0.05)。免疫组化结果显示,胰腺癌组织中mir-155、mir-196a、mir-181a、mir-181b的表达明显高于其在正常胰腺组织中的表达(P<0.05)。结论胰腺癌细胞系和胰腺癌组织中均高表达mir-155、mir-196a、mir-181a、mir-181b,说明其与胰腺癌的发生、发展有密切关系,这些特异性的MieroRNAs有望成为胰腺癌早期诊断的标记物,有极高的诊断价值和广阔的临床应用前景。
Objective To analyze the clinical value of microRNAs applied in the early diagnosis of pancreatic cancer.Methods The expression of mir-155,mir-196a,mir-181a,mir-181b in three pancreatic cancer cell lines PANC-1,PaCa-2,AsPC-1 pancreatic cancer,normal pancreatic cells and plasmas of 100 patients with pancreatic cancer ,100 normal volunteers were detected by Real-time PCR,and the results were analyzed.The expression of mir-155,mir-196a,mir-181a,mir-181b in 100 pancreatic cancer tissues and 100 normal pancreatic tissues were detected by immunohistochemical,and the results were analyzed. Results Real-timePCR test results showed that compared with normal pancreatic cells,mir-155,mir-196a expression in three pancreatic cancer cells PANC-1,PaCa-2,AsPC-1 were significantly increased(P<0.05).Compared with healthy volunteers,mir-155,mir-196a,mir-181a,mir-181b expression of plasma in pancreatic cancer patients were significantly higher(P<0.05).Immunohistochemistry results showed that mir-155,mir-196a mir-181a and mir-181b expression in pancreatic tissue were significantly higher than that in normal pancreatic tissue (P <0.05 ).Conclusion The expression of mir-155,mir-196a,mir-181a and mir-181b are high in pancreatic cancer tissues and pancreatic cancer cell lines,which are closely related to the development of pancreatic cancer.Those microRNAs are expected to be cancer markers for early diagnosis of,pancreatic cancer,have highly diagnostic value and broad clinical application.
出处
《中国生化药物杂志》
CAS
北大核心
2014年第3期52-54,共3页
Chinese Journal of Biochemical Pharmaceutics
基金
江苏省卫生厅中青年扶持计划(JW-2010BNO.019)
