摘要
The bovine embryos used for nonsurgical transfer should be morulae or blastocysts which are generally obtained from donor cow through superovulation and insemination. Due to the high cost of the embryos, the spread of embryo transfer is influenced to certain extent. In recent years, in vitro fertilization (IVF)technique has been adopted abroad to expand the source of embryos. The methods employed normally were culturing the embryos in rabbit’s or sheep’s oviducts (in vivo culture) to overcome '8—16-cell block', the morula or blastocyst developed in vivo was recovered and then transferred to recipients. However, the research concerning in vitro culture of IVF ova to the morula-blastocvst stage was
