摘要
本研究用ABC免疫染色法,结合葡萄糖氧化酶-DAB-硫酸镍铵(Glucose oxidase-DAB-Nickle,GDN)显色技术,在Bouin液固定的常规石蜡切片上,研究了5-羟色胺(5-hydroxytryptamin,5-HT)在豚鼠胰腺内的定位和分布,并用相邻切片免疫双标记,观察了它与胰岛素的共存关系,结果发现,在豚鼠胰腺内,外分泌部均有5-HT免疫反应细胞分布。在胰腺内分泌部(胰岛)5-HT免疫反应细胞分布均匀,大部分胰岛细胞呈阳性5-HT样免疫反应,用相邻薄切片免疫双标记技术证明,胰岛内的5-HT免疫反应细胞主要是B细胞。在胰腺外分泌部,5-HT免疫反应细胞呈单个分散或聚集分布,主要位于腺泡和导管等处,偶见于结缔组织间隔中。本文对研究5-HT在胰腺的生理作用及其机制提供了形态学依据。
The localization and distribution of 5-HT in guinea-pig pancreas were investigated with ABC immunostaining method combined with the glucose oxidase-DAB-Nickle developing technique on paraffin sections. In this study, It was demonstrated that 5-HT immunoreactive cells were present both in the endocrine (islets)and exocrine pancreas of the guinea pig. The 5-HT immunoreactive cells in the pancreatic islets were identical in distribution and multiformed in shape. The majority of islets cells showed positive 5-HT immunostaining. The coexistence of 5-HT and insulin in the pancreatic islets was also proved with immunostaining of adjacent thin sections. 5-HT immunoreactive cells were also found in the epithelium of the ducts and acini of the exocrine pancreas. The significance of these results needs farther study.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1992年第2期143-146,259,共5页
Chinese Journal of Histochemistry and Cytochemistry
关键词
5-羟色胺
胰腺
免疫组织化学
豚鼠
5-Hydroxytryptamin
Pancreas
Immunohistochemistry
Gguinea-pig
