摘要
采用 P_(21)单抗 SCI-oncogemaI,用抗生物素-生物素-过氧化酶复合物技术(ABC 法),检测了141例胃癌、重度不典型增生、肠化生、萎缩性胃炎、浅表性胃炎及正常胃粘膜 ras 癌基因蛋白产物 P_(21)在单个细胞水平的表达。结果示53例胃癌 ras P_(21)表达多出现于早期胃癌。阳性率为87%,中晚期癌为阳性率胃74%,重度不典型增生为86%,萎缩性胃炎为74%,肠化生为73%,正常胃粘膜仅1例呈弱阳性.本研究提示癌前病变、早期胃癌时 ras 癌基因大量激活,其功能性蛋白产物 P_(21)过度表达,与胃癌的早期癌变可能有密切关系.
At present,the products of oncogenes that has been studied extensively is the ras family which encodes a group of closely related 21000 dalton proteins.The present paper reported the expression of the ras oncogene product P_(21) in 141 gastric carcinoma,severe dysplasia,metaplasia,atrophic gastritis,superficial gastritis,and normal gastric mucosa.The analysis was per- formed on paraffin sections by an immunohistochemical method using P_(21) monoclonal antibody SCI-oncogema Ⅰ.The results showed that P_(21) was ex- pressed with positive stained cells in 87% in early gastric carcinoma.In advan- ced gastric carcinoma,dysplasia and metaplasia,positive stained cells were 74%, 86% and 73%,respectively.It shows that the overexpression of products of oncogenes has appeared in the precancerous lesions.The results of this inves- tigation suggest that ras P_(21) overexpression may be related to early events of gastric carcinogenesis.
出处
《医学研究生学报》
CAS
1992年第1期6-8,88,共4页
Journal of Medical Postgraduates
关键词
癌基因
胃癌
免疫组织化学
oncogene
gastric cancer
immunohistochemistry
