摘要
本文对不同封闭液及其稀释液、不同显影时间、不同方法保存的金标记抗体对斑点免疫金银染色检测日本血吸虫抗体实验结果的影响进行了探讨。认为以适当浓度的羊血清/BSA作封闭液,小牛血清作稀释液较好;20℃显影20min为宜;金标抗体以不加NaN_3—20℃保存较佳,有效期可达2年以上。
By using different blocking and diluting fluids, different developing time and different gold-labeled anti-immunoglobulin (GLA-Ig) which had been preserved in different conditions, the influence on the dot-immunogold-siiver staining (Dot-IGSS) for diagnosis of schis-tosomiasis japonica was studied. The results suggested that using suitable amount of sheep/ bovine serum albumin (BSA) as blocking fluid and calf serum as diluting fluid was a good choice, and 20℃ 20 min was the appropriate time for development. The GLA-Ig without Na3N3 could be preserved in - 20℃ for 2 years if it was not frozen and melted repeatedly.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
1992年第4期218-220,共3页
Chinese Journal of Schistosomiasis Control
关键词
斑点免疫金银染色
日本血吸虫
抗体检测
影响因素
dot-immunogold-silver staining (Dot-IGSS)
schistosoma japonicum
antibody assay
influential factor
