摘要
以24株人肠道病原菌(包括病原性大肠杆菌12株、沙门氏菌8株、志贺氏菌3株、小肠结肠炎耶尔森氏菌1株)作为抗原,对乳牛进行系统免疫,免疫乳与非免疫乳乳中IgG含量无显著差异。系统免疫并不增加乳中IgG的含量,但IgG的抗体特异性大大增强,所得的免疫初乳中乳抗体对24种不同病原菌的凝集价为28～212,为普通初乳中乳抗体凝集价的32-256倍。采用硫酸胺盐析从免疫初乳中分离乳抗体,免疫初乳中的特异性IgG乳抗体较普通初乳中的IgG可显著抑制大肠杆菌和沙门氏菌的生长。动物试验表明,免疫乳中特异性的IgG对大肠杆菌和沙门氏菌所致小鼠腹泻具有很好的保护作用,而普通乳中非特异性的IgG则无此作用,这是由于IgG的特异性所决定。
strains of pathogenic bacteria (including 12 of pathogenic E.coli,8 o f Salmonella,3 of Shigella and 1 of Yersinia enterocolitica) originated from hu man intestinal tract were selected as antigens,and dairy cows were immunized.Va ccination could not increase the concentration of IgG in the immune milk,but si gnificantly highten the specificity of mik antibodies.The antibody titers agains t 24 pathogenic bacteria in immune colostrum were 28～212,32～256 folds of cont rol regular colostrum. Comparing to the IgG isolated from regular colostrum,the specific IgG isolated from immune colostrum could significantly inhibit the gro wth of E.coli and Salmonella in vitro,and could protect mice from diarrhea chal lenged with E.coli and Salmonella.
出处
《中国乳品工业》
CAS
北大核心
2004年第6期3-7,共5页
China Dairy Industry
基金
国家自然科学基金资助项目(29366012
29766004)
