摘要
作者用反相高效液相色谱柱前OPA衍化法[1]，对12例正常人血浆及12例老年性白内障患者血浆与房水的游离氨基酸进行定量分析，血浆和房水均能检出24种游离氨基酸。并把老年性白内障血浆和房水的各种游离氨基酸进行比较，发现老年性白内障的血浆和房水中，有19种游离氨基酸的浓度有显著性差异（P＜0．01）。房水中有12种游离氨基酸浓度较血浆浓度显著下降，且大多数为亲水性氨基酸，有7种游离氨基酸浓度较血浆浓度显著增高。且大多数为疏水性氨基酸。讨论了这些游离氨基酸浓度的变化与营养、血－房水屏障以及蛋白质结构的关系。探讨老年性白内障形成的可能原因。
We determined the concentrations of 24 free amino acids in aqueous humor and plasma of 12 patients with senile cataract and 12 healthy subjects as control. Amino acid analysis was carried out by reversed-phase high performance liquid chromatography after pre-column derivatization with O-pathalaldehyde. Concentrations of 19 amino acids in aqueous humor of the senile cataract patients were lower than those in their plasma. Among them 12 were lower at P<0. 01: Asp, His, Cit, 3-MHis, Tau, Try, Orn, Glu, Ser, Gly, Ala and Lys.The majority of them were hydrophilic amino acids. While 7 other amino acids, most of them hydrophobic, including sulphur-containing amino acids, had concentrations higher in humour than in plasma at P<0. 01: Cys, Tyr, Met, Gln, Val, Phe and Leu. Possible relationship of these difference in amino acid concentrations with nutrition, blood-aqueous barrier and primary structure of proteins present in humour were discussed. Based on these findings a hypothesis on the pathogenesis of senlie cataract was proposed.
出处
《广州医学院学报》
1994年第4期62-66,70,共6页
Academic Journal of Guangzhou Medical College
