摘要
本文提出了荧光法测定强力霉素(DC)的新方法。强力霉素碱性降解后与铝离子形成强荧光的络合物,并据此进行荧光测定,其荧光强度在4.0×10^(-8)～7.0×10^(-6)mol/L 范围内与强力霉素浓度成正比,共存的其它四环素类抗菌素不干扰强力霉素的测定,测定的检测限为3.0×10^(-8)mol/L。此法用于血清和尿液中强力霉素的测定,回收率分别为93～97%和91%～99%。
A new fluorimetric method for the determination of trace doxycycline is proposed. The Alkaline degradation product of doxycycline(isodoxycycline)reacts rapidly with AI^(3+) and produces strong fluorescent chelate which is detected by fluorimetry. The fluorescence intensity of the chelate is proportional to the concentration of doxycyctine over the range of 4.0×10^(-8)～7.0×10^(-6)mol/L.Coexisting methacyctine and other tetracyclines do not inter- fere in the determination of doxycycline.The detection limits is 3.0×10^(-8)mol/L.This method has been used for the determination of doxycycline in serum and urine with 93%～ 97% and 91%～99% recoveries,respectively.
出处
《福建分析测试》
CAS
1996年第2期459-462,共4页
Fujian Analysis & Testing
基金
福建省自然科学基金
