摘要
目的通过检测射线照射前后鼻咽癌CNE1细胞多药耐药基因(mdr1基因)及其编码产物P糖蛋白(P-gp)的表达和功能为临床鼻咽癌放化疗顺序提供参考。方法利用RT-PCR、Western blotting和流式细胞仪检测射线照射前后CNE1细胞的mdr1基因和P-gp的表达及对柔红霉素的外排功能。结果鼻咽癌CNE1细胞射线照射前mdr1基因、P-gp不表达;照射后较长时间内mdr1基因、P-gp均明显表达;对柔红霉素的摄取较射线照射前低。结论鼻咽癌CNE1细胞射线照射后化疗敏感性降低,提示临床对于中晚期鼻咽癌的治疗应考虑先化疗再放疗即诱导化疗的治疗方案。
Objective To examine the changes in the function and expression of multidrug resistance gene (mdr1) and P-gly- coprotein (P-gp) in nasopharyngeal carcinoma (NPC) CNE1 cell following irradiation for determining the sequential order of radiotherapy and chemotherapy in the treatment of NPC. Methods The expressions of mdr1 gene and its protein P-gp as well as the function of P-gp efflux were examined in CNE1 cells before and after irradiation exposure by reverse transcriptional polymerase chain reaction (RT-PCR), Western blotting and flow cytometry, respectively. Results Irradiation of CNE1 cells induced a long-term overexpression of mdr1 gene and P-gp and reduction in intracellular daunorubicin accumulation. Conclusion Irradiation decreases the chemotherapy sensitivity of CNE1 cell, and induction chemotherapy should be therefore performed before radiotherapy in the treatment of advanced NPC.
出处
《第一军医大学学报》
CSCD
北大核心
2004年第6期646-649,共4页
Journal of First Military Medical University
基金
国家自然科学基金(30270424)~~
关键词
X射线照射
鼻咽癌
多药耐药基因
表达
癌细胞
nasopharyngeal neoplasm
tumor cells, cultured
drug tolerance
X-rays
P-glycoproteins
