摘要
本研究借助免疫电镜和溃变纤维电镜追踪等技术，探索我国南方特产，昼行动物“灵长类原宗”树鼠句（ＴＢＣ）下丘脑视交叉上核（ＳＣＮ）的突触，特别是ＳＣＮ中５－羟色胺（５－ＨＴ）能和视纤维传入的突触联系的超微结构特征，结果表明：ＴＢＣ的ＳＣＮ主要包含不对称性ＧｒａｙⅠ型（ＧＴⅠ）和对称性ＧｒａｙⅡ型（ＧＴⅡ）两种突触类型。其中ＧＴⅠ突触的后膜下颇多呈现“三体一线”的节下小体。５－ＨＴ能末梢在ＴＢＣ的ＳＣＮ（主要在其腹侧）中形成丰富的ＧＴⅠ和ＧＴⅡ突触。还观察到ＳＣＮ中５－ＨＴ能末梢和非５－ＨＴ能轴突构成颇多的轴－轴突触，５－ＨＴ能末梢既可为突触前也可为突触后成分。这在大鼠ＳＣＮ中未见报道。
The present study was undertaken to explore the ultrastructural characteristics of synapses, especially the 5 HT ergic and retinal afferent synaptic connections in the hypothalamic suprachiasmatic nucleus(SCN) of Tupaia belangeri chinensis(TBC), the unique native of South Chinas diurnal “primitive stock” which is more close to the Primates. By using the methods of immuno-electro-microscopic and degeneration tracing techniques, we found that two types of synapses-asymmetrical Gray-Type I-(GTⅠ) and symmetrical Gray-Type Ⅱ-(GTⅡ) synapses could be seen in TBCs SCN; considerable ammount of Subjunctional bodies(sb) could be observed beneath the postsynaptic membrane of GTI synapses. The 5-HT-ergic terminals made abundant GTⅠ and GTⅡ synaptic contacts with the dendrites in TBCs SCN (mainly its ventral portion). It was also worthy of noting that relatively abundant 5-HT-ergic terminals in TBCs SCN made synaptic contacts with non-5-HT-ergic axones to form axoaxonal synapses, and the 5-HT-ergic terminals could be either presynaptic or postsynaptic, which had not been reported in rats SCN hitherto. Degenerating retinal afferents mainly made GTI synapses in the ventrolateral part of TBCs SCN.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
1997年第1期24-29,共6页
Chinese Journal of Histochemistry and Cytochemistry
基金
国家自然科学基金
